Functional characteristics of bone marrow stromal cells in patients with acute leukemia

Pathogenesis of acute leukemia is associated with the presence of genetic and functional aberrations in hematopoietic stem cells. However, as one of the possible factors contributing to the progression of disease could be regarded as the pull of the stromal cells of bone marrow. In this paper we evaluated the major functional characteristics of stromal cells: proliferative activity, hematopoiesis supportive and differentiation ability, as well as analysis of the expression of adhesion molecules. Stromal cells derived from acute leukemia patients in comparison with healthy donors are characterized by a high proliferative activity and a pronounced ability to osteogenic and adipogenic differentiation with prominent expression of hematopoietic stem cells homing mediating molecules.

стромальные клетки костного мозга, острые лейкозы, функциональные характеристики, bone marrow stromal cells, acute leukemia, functional characteristics

1. Афанасьев Б.В., Кулибаба Т.Г., Забелина Т.С. и др. Клонирование кроветворных клеток больных с различными формами гемопоэтических дисплазий в агаре (клинико-культуральные сопоставления) // Терапевт. арх. - 1982. - № 8. - С. 97- 103.

2. Blau O., Hofmann W.K., Baldus C.D. et al. Chromosomal aberrations in bone marrow mesenchymalstroma cells from patients with myelodysplastic syndrome and acute myeloblastic leukemia // Exp. Hematol. - 2007. - № 35 (2). - Р. 221-229.

3. Borojevic R.L., Roela R.A., Rodarte R.S. et al. Bone marrow stroma in childhood myelodysplastic syndrome: composition, ability to sustain hematopoiesis in vitro, and altered gene expression // Leuk. Res. - 2004. - № 28 (8). - Р. 831-844.

4. Chamberlain G.L., Fox J., Ashton B., Middleton J. Concise review: mesenchymal stem cells: their phenotype, differentiation capacity, immunological features, and potential for homing // Stem. Cells. - 2007. - № 25 (11). - Р. 2739 - 2749.

5. Flores-Figueroa E., Arana-Trejo R.M., Gutierrez-Espindola G. et al. Mesenchymal stem cells in myelodysplastic syndromes: phenotypic and cytogenetic characterization // Leuk. Res. - 2005. - № 29 (2). - Р. 215 - 224.

6. Friedenstein A.J., Chailakhyan R.K., Latsinik N.V. et al. Stromal cells responsible for transferring the microenvironment of the hemopoietic tissues. Cloning in vitro and retransplantation in vivo // Transplantation. - 1974. - № 17 (4). - Р. 331 - 340.

7. Gunsilius E., Duba H.C., Petzer A.L. et al. Evidence from a leukaemia model for maintenance of vascular endothelium by bone-marrow-derived endothelial cells // Lancet. - 2000. - № 355 (9216). - P. 1688-1691.

8. Konopleva M., Tabe Y., Zeng Z., Andreeff M. Therapeutic targeting of microenvironmental interactions in leukemia: mechanisms and approaches // Drug Resist. Updat. - 2009. - № 12 (4 - 5). - Р. 103-113.

9. Le Blanc K., Samuelsson H., Gustafsson B. et al. Transplantation of mesenchymal stem cells to enhance engraftment of hematopoietic stem cells // Leukemia. - 2007. - № 21 (8). - Р. 1733-1738.

10. Le Blanc K. Mesenchymal stromal cells: Tissue repair and immune modulation // Cytotherapy. - 2006. - № 8 (6). -Р. 559-561.

11. Podar K., Richardson P.G., Hideshima T. et al. The malignant clone and the bone-marrow environment // Best Pract. Res. ClinHaematol. - 2007. - № 20 (4). - Р. 597-612.

12. Prockop D.J. Marrow stromal cells as stem cells for non-hematopoietic tissues // Science. - 1997. - Vol. 4. - № 276 (5309). - Р. 71-74.

13. Scadden D.T. The stem cell niche in health and leukemic disease // Best Pract. Res. ClinHaematol. - 2007. - № 20 (1). -Р. 19-27.