FABP4 gene expression in subcutaneous and visceral adipose tissue in patients with obesity and type 2 diabetes mellitus
K. V. Dracheva,
I. A. Pobozheva,
K. A. Anisimova,
Z. M. Hamid,
A. P. Sapojnikova,
S. G. Balandov,
D. I. Vasilevsky,
S. N. Pchelina,
V. V. Miroshnikova https://doi.org/10.24884/1607-4181-2022-29-1-46-53
Abstract
Introduction. Obesity is associated with a high risk of developing concomitant diseases such as: metabolic syndrome, type 2 diabetes mellitus (DM2), cardiovascular pathology. FABP4 (fatty acid binding protein) is the specific lipid chaperone and an important protein for the function of adipose tissue and is one of the adipocytokines secreted by adipose tissue.
The objective of the study was to investigate the FABP4 gene expression in subcutaneous and visceral adipose tissue (SAT and VAT) in patients with obesity and DM2.
Methods and materials. SAT and VAT samples were obtained during bariatric surgery (N=43, BMI>35): obese with DM2 (N=21), obese without DM2 (N=22). Samples for the control group without obesity (N=15, BMI<30) were obtained during planned operations on the abdominal cavity. FABP4 mRNA level was estimated by real-time PCR.
Results. It has been demonstrated that the mRNA level of the FABP4 gene in SAT and VAT is reduced in obesity, regardless of the manifestation of DM2 (p<0.01). A negative correlation was observed between the mRNA level of the FABP4 gene in adipose tissue and the BMI index (for SAT: r=—0.327, p = 0.016; for VAT: r=—0.304, p = 0.024).
Conclusion. The expression level of FABP4 gene in AT can act as a marker of AT dysfunction in obesity.
Supporting agencies: This work was supported by the grant from the Russian foundation for basic research RFBR № а 20-015-00502.
About the Authors
K. V. Dracheva
Pavlov University; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Competing Interests:
Russian Federation
Kseniia V. Dracheva - Postgraduate Student, Laboratory Assistant at the Laboratory of Medical Genetics of the Department of Molecular, Genetic, and Nanobiological Technologies of the Research Center, Pavlov University; Laboratory Assistant at the Laboratory of Human Molecular Genetics, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute».
Saint Petersburg; Gatchina.
Competing Interests:
No conflict of interest
I. A. Pobozheva
Pavlov University; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Competing Interests:
Russian Federation
Irina A. Pobozheva - Junior Research Fellow at the Laboratory of Medical Genetics of the Department of Molecular, Genetic, and Nanobiological Technologies of the Research Center, Pavlov University; Junior Research Fellow at the Laboratory of Human Molecular Genetics, Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre «Kurchatov Institute».
Saint Petersburg; Gatchina.
Competing Interests:
No conflict of interest
K. A. Anisimova
Pavlov University
Russian Federation
Competing Interests:
Russian Federation
Kristina A. Anisimova - Surgeon, Surgical Department № 2 of the Research Institute of Surgery and Emergency Medicine, Pavlov University.
Saint Petersburg.
Competing Interests:
No conflict of interest
Z. M. Hamid
Pavlov University
Russian Federation
Competing Interests:
Russian Federation
Zarina M. Hamid - Surgeon, Surgical Department № 2 of the Research Institute of Surgery and Emergency Medicine, Pavlov University.
Saint Petersburg.
Competing Interests:
No conflict of interest
A. P. Sapojnikova
Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Competing Interests:
Russian Federation
Antonina P. Sapojnikova - Laboratory Assistant, Petersburg Nuclear Physics Institute named by B. P. Konstantinov of National Research Centre «Kurchatov Institute».
Gatchina.
Competing Interests:
No conflict of interest
S. G. Balandov
Pavlov University
Russian Federation
Competing Interests:
Russian Federation
Stanislav G. Balandov - Cand. of Sci. (Med.), Head of the Surgical Department № 2, Pavlov University.
Saint Petersburg.
Competing Interests:
No conflict of interest
D. I. Vasilevsky
Pavlov University
Russian Federation
Competing Interests:
Russian Federation
Dmitry I. Vasilevsky - Dr. of Sci. (Med.), Professor of the Department of Faculty Surgery with a Course of Laparoscopic Surgery and Cardiovascular Surgery, Pavlov University.
Saint Petersburg.
Competing Interests:
No conflict of interest
S. N. Pchelina
Pavlov University; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Competing Interests:
Russian Federation
Sofya N. Pchelina - Dr. of Sci. (Biol.), Head of the Department of Molecular, Genetic, and Nanobiological Technologies of the Research Center, Pavlov University; Head of the Laboratories of Human Molecular Genetics, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute».
Saint Petersburg; Gatchina.
Competing Interests:
No conflict of interest
V. V. Miroshnikova
Pavlov University; Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute»
Russian Federation
Competing Interests:
Russian Federation
Valentina V. Miroshnikova - Cand. of Sci. (Biol.), Head of the Laboratory of Medical Genetics of the Department of Molecular, Genetic, and Nanobiological Technologies of the Research Center, Pavlov University; Senior Research Fellow at the Laboratory of Human Molecular Genetics, Petersburg Nuclear Physics Institute named by B.P. Konstantinov of National Research Centre «Kurchatov Institute».
6-8, L'va Tolstogo str., Saint Petersburg, 197022.
Competing Interests:
No conflict of interest
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For citations:
Dracheva K.V., Pobozheva I.A., Anisimova K.A., Hamid Z.M., Sapojnikova A.P., Balandov S.G., Vasilevsky D.I., Pchelina S.N., Miroshnikova V.V. FABP4 gene expression in subcutaneous and visceral adipose tissue in patients with obesity and type 2 diabetes mellitus. The Scientific Notes of the Pavlov University. 2022;29(1):46-53. (In Russ.) https://doi.org/10.24884/1607-4181-2022-29-1-46-53
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