Цитотоксичность леканоровой кислоты, выделенной из лишайника Parmotrema tinctorium

Широко распространённый на территории России лишайник Parmotrema tinctorium (Despr. ex Nyl.) Hale накапливает в сердцевинном слое таллома орцинольный депсид ­– леканоровую кислоту, потенциально обладающей биологической активностью. В настоящем исследовании мы выделили и охарактеризовали леканоровую кислоту и оценили ее цитотоксические свойства. Структура леканоровой кислоты была подтверждена на основе спектральных данных (ЯМР, МС и ИК). Цитотоксическая активность выделенных соединений была оценена в отношении раковых (Panc-1, A549, Т98G, HeLa) и нормальной (HEK293) линий клеток человека с помощью МТТ-теста. Показано, что леканоровая кислота проявила умеренную цитотоксичность в отношении линии карциномы шейки матки HeLa со значениями IC₅₀ = 121 мкмоль. Результаты проведенного докинга и молекулярного моделирования указывают на потенциальное связывание леканоровой кислоты с белками AKT1 и PI3Kα. Эти результаты подчеркивают потенциал лишайникового депсида, в качестве источника противораковых препаратов и требуют дальнейшего изучения механизмов его действия.

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