uzspbgmuУчёные записки Первого Санкт-Петербургского государственного медицинского университета имени академика И. П. ПавловаThe Scientific Notes of the Pavlov University1607-41812541-8807Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1607-4181-2025-32-4-103-115uzspbgmu-1219Research ArticleОРИГИНАЛЬНЫЕ РАБОТЫORIGINAL PAPERSЦитотоксичность леканоровой кислоты, выделенной из лишайника Parmotrema tinctoriumCytotoxicity of lecanoric acid isolated from the lichen Parmotrema tinctoriumhttps://orcid.org/0000-0001-8755-7140ПрокопьевИ. А.ProkopyevI. A.

Прокопьев Илья Андреевич, кандидат биологических наук, старший научный сотрудник

197022, Санкт-Петербург, ул. Профессора Попова, д. 2;

197758, Санкт-Петербург, поселок Песочный, ул. Ленинградская, д. 70

Prokopyev Ilya A., Cand. of Sci. (Biol.), Senior Research Fellow

2, Professora Popova str., Saint Petersburg, 197022;

70, Leningradskaya str., Pesochny settlement, Saint Petersburg, 197758

prokopiev@binran.ruМинасянА. Г.MinasyanA. G.

Минасян Арман Гаврушевич, соискатель кафедры общей и биоорганической химии

197022, Санкт-Петербург, ул. Льва толстого, д. 6-8

Minasyan Arman G., Candidate of the Department of General and Bioorganic Chemistry

6-8, L’va Tolstogo str., Saint Petersburg, 197022

https://orcid.org/0000-0003-3060-2232ШемчукО. С.ShemchukO. S.

Шемчук Ольга Сергеевна, аспирант, специалист по учебно-методической работе кафедры общей и биоорганической химии; научный сотрудник отдела фундаментальных исследований

197758, Санкт-Петербург, поселок Песочный, ул. Ленинградская, д. 70;

197022, Санкт-Петербург, ул. Льва толстого, д. 6-8

Shemchuk Olga S., Postgraduate Student, Specialist in Educational and Methodological Work of the Department of General and Bioorganic Chemistry

70, Leningradskaya str., Pesochny settlement, Saint Petersburg, 197758;

6-8, L’va Tolstogo str., Saint Petersburg, 197022

https://orcid.org/0009-0001-8840-2455ПетуховаН. В.PetukhovaN. V.

Петухова Наталья Витальевна, кандидат биологических наук, доцент кафедры общей и биоорганической химии

197758, Санкт-Петербург, поселок Песочный, ул. Ленинградская, д. 70;

197022, Санкт-Петербург, ул. Льва толстого, д. 6-8

Petukhova Natalia V., Cand. of Sci. (Biol.), AssociateProfessor of the Department of General and Bioorganic Chemistry

70, Leningradskaya str., Pesochny settlement, Saint Petersburg, 197758;

6-8, L’va Tolstogo str., Saint Petersburg, 197022

https://orcid.org/0009-0003-8585-2746КременецкаяУ. А.KremenetskayaU. A.

Кременецкая Ульяна ОТЧЕСТВО, студент биологического факультета

199034, Санкт-Петербург, Университетская набережная, д. 7-9

Kremenetskaya Uliana A., Student of the Faculty of Biology

7-9, Universitetskaya emb., Saint Petersburg, 199034

https://orcid.org/0000-0002-0463-7725МиколайчукО. В.MikolaichukO. V.

Миколайчук Ольга Владиславовна, кандидат химических наук, старший научный сотрудник отдела фундаментальных исследований

197758, Санкт-Петербург, поселок Песочный, ул. Ленинградская, д. 70

Mikolaichuk Olga V., Cand. of Sci. (Chem.), Senior Research Fellow of the Department of Fundamental Researches

70, Leningradskaya str., Pesochny settlement, Saint Petersburg, 197758

https://orcid.org/0000-0003-3330-6324МолчановО. Е.MolchanovO. E.

Молчанов Олег Евгеньевич, доктор медицинских наук, руководитель отдела фундаментальных исследований

197758, Санкт-Петербург, поселок Песочный, ул. Ленинградская, д. 70

Molchanov Oleg E., Dr. of Sci. (Med.), Head of the Department of Fundamental Researches

70, Leningradskaya str., Pesochny settlement, Saint Petersburg, 197758

https://orcid.org/0000-0001-8174-7461МайстренкоД. Н.MaistrenkoD. N.

Майстренко Дмитрий Николаевич, доктор медицинских наук, директор

197758, Санкт-Петербург, поселок Песочный, ул. Ленинградская, д. 70

Maistrenko Dmitrii N., Dr. of Sci. (Med.), Director

70, Leningradskaya str., Pesochny settlement, Saint Petersburg, 197758;

7-9, Universitetskaya emb., Saint Petersburg, 199034

https://orcid.org/0000-0003-2239-2044СеменовК. Н.SemenovK. N.

Семёнов Константин Николаевич, доктор химических наук, профессор, зав. кафедрой общей и биоорганической химии

197022, Санкт-Петербург, ул. Льва толстого, д. 6-8;

199034, Санкт-Петербург, Университетская набережная, д. 7-9

Semenov Konstantin N., Dr. of Sci. (Chem.), Professor, Head of the Department of General and Bioorganic Chemistry

6-8, L’va Tolstogo str., Saint Petersburg, 197022;

7-9, Universitetskaya emb., Saint Petersburg, 199034

https://orcid.org/0000-0002-3717-0471ШаройкоВ. В.SharoykoV. V.

Шаройко Владимир Владимирович, доктор биологических наук, профессор кафедры общей и биоорганической химии

197022, Санкт-Петербург, ул. Льва толстого, д. 6-8;

199034, Санкт-Петербург, Университетская набережная, д. 7-9

Sharoyko Vladimir V., Dr. of Sci. (Biol.), Professor of the Department of General and Bioorganic Chemistry

6-8, L’va Tolstogo str., Saint Petersburg, 197022

Ботанический институт им. В. Л. Комарова РАН; Российский научный центр радиологии и хирургических технологий имени академика A. M. ГрановаРоссияKomarov Botanical Institute of the Russian Academy of Sciences; Granov Russian Research Center of Radiology and Surgical TechnologiesRussian FederationПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаРоссияPavlov UniversityRussian FederationРоссийский научный центр радиологии и хирургических технологий имени академика A. M. Гранова; Первый Санкт-Петербургский государственный медицинский университет имени академика И. П. ПавловаРоссияGranov Russian Research Center of Radiology and Surgical Technologies; Pavlov UniversityRussian FederationСанкт-Петербургский государственный университетРоссияSt. Petersburg State UniversityRussian FederationРоссийский научный центр радиологии и хирургических технологий имени академика A. M. ГрановаРоссияGranov Russian Research Center of Radiology and Surgical TechnologiesRussian FederationПервый Санкт-Петербургский государственный медицинский университет имени академика И. П. Павлова; Санкт-Петербургский государственный университетРоссияPavlov University; St. Petersburg State UniversityRussian Federation202508042026324103115Copyright © Прокопьев И.А., Минасян А.Г., Шемчук О.С., Петухова Н.В., Кременецкая У.А., Миколайчук О.В., Молчанов О.Е., Майстренко Д.Н., Семенов К.Н., Шаройко В.В., 20262026Прокопьев И.А., Минасян А.Г., Шемчук О.С., Петухова Н.В., Кременецкая У.А., Миколайчук О.В., Молчанов О.Е., Майстренко Д.Н., Семенов К.Н., Шаройко В.В.Prokopyev I.A., Minasyan A.G., Shemchuk O.S., Petukhova N.V., Kremenetskaya U.A., Mikolaichuk O.V., Molchanov O.E., Maistrenko D.N., Semenov K.N., Sharoyko V.V.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sci-notes.ru/jour/article/view/1219

Широко распространённый на территории России лишайник Parmotrema tinctorium (Despr. ex Nyl.) Hale накапливает в сердцевинном слое таллома орцинольный депсид ­– леканоровую кислоту, потенциально обладающей биологической активностью. В настоящем исследовании мы выделили и охарактеризовали леканоровую кислоту и оценили ее цитотоксические свойства. Структура леканоровой кислоты была подтверждена на основе спектральных данных (ЯМР, МС и ИК). Цитотоксическая активность выделенных соединений была оценена в отношении раковых (Panc-1, A549, Т98G, HeLa) и нормальной (HEK293) линий клеток человека с помощью МТТ-теста. Показано, что леканоровая кислота проявила умеренную цитотоксичность в отношении линии карциномы шейки матки HeLa со значениями IC50 = 121 мкмоль. Результаты проведенного докинга и молекулярного моделирования указывают на потенциальное связывание леканоровой кислоты с белками AKT1 и PI3Kα. Эти результаты подчеркивают потенциал лишайникового депсида, в качестве источника противораковых препаратов и требуют дальнейшего изучения механизмов его действия.

The widespread in Russia lichen Parmotrema tinctorium (Despr. ex Nyl.) Hale accumulates orcinolic depsid – lecanoric acid in the core layer of the thallus, which potentially has biological activity. In the present study, we isolated and characterized lecanoric acid and evaluated its cytotoxic properties. The structure of lecanoric acid was confirmed based on spectral data (NMR, MS, and IR). The cytotoxic activity of the isolated compounds was assessed against cancer (Panc-1, A 549, T98G, HeLa) and normal (HEK293) human cell lines using the MTT assay. It was shown that lecanoric acid exhibited moderate cytotoxicity against the HeLa cervical carcinoma cell line with IC50 values of 121 μmol. The results of the performed docking and molecular modeling indicate the potential binding of lecanoric acid to AKT 1 and PI3Kα proteins. These results highlight the potential of lichen depside as a source of anticancer drugs and require further study of its mechanisms of action.

Parmotrema tinctoriumлеканоровая кислотадепсидыцитотоксичностьпротивораковая активностьParmotrema tinctoriumlecanoric aciddepsidescytotoxicityanticancer activityРабота выполнена при финансовой поддержке Министерства здравоохранения Российской Федерации (государственное задание «Выделение и изучение противоопухолевой активности вторичных метаболитов (алифатических кислот, депсидов, депсидонов, дибензофуранов) растительного происхождения». Регистрационный номер ЕГИСУ: 1023022200057-2-3.2.21.).The work was carried out with the financial support of the Ministry of Health of the Russian Federation (state assignment «Isolation and study of antitumor activity of secondary metabolites (aliphatic acids, depsides, depsidones, dibenzofurans) of plant origin». USHIS registration number: 1023022200057-2-3.2.21.).ReferencesMolnár K., Farkas E. 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The authors declare that there are no conflicts of interest present.