The effect of fish oil and ozonated fish oil on the oxidative status of animals during simulation of physical load

The objective was to study the effectiveness of fish oil (FO) and ozonated fish oil (OFO) on the intensity of lipid peroxidation (LPO) processes and the antioxidant system of the blood during physical activity «to failure».

Methods and materials. The animals were divided into 4 groups of 12 rats. Saline was administered orally to control animals (group 1). Rats (group 2) were fed fish oil (dose 35 mg/kg), rats of group 3 – ozonated fish oil (dose 35 mg / kg, ozonide number 3000), group 4 – ozonated fish oil (dose 35 mg/kg, ozonide number 1500). Physical activity was modelated the method of forced swimming of rat «to failure» with a load of 10 % of body weight. The state of the LPO system was assessed by the concentration of malondialdehyde (MDA) in erythrocytes and the level of diene conjugates (DC), triene conjugates (TC) and Schiff bases (SH) in the blood plasma. The state of the antioxidant system was determined by the activity of catalase in the blood plasma.

Results. Physical activity had an increase in DC, TC, OR in the blood plasma, an increase in the MDA content in erythrocytes, which was accompanied by a gradual increase in catalase activity in the blood plasma. The administration of FO against the background of physical activity determined less pronounced lipid peroxidation, while the introduction of OFO with an ozonide number of 3000 determined the most pronounced lipid peroxidation compared to the control. The lowest oxidative effect of physical compounds was recorded with the introduction of OFO with an ozonide number of 1500.

Conclusions. Oral administration of OFO with an ozonide number of 1500 during physical exercise of significant intensity inhibited the development of oxidative stress against the background of high antioxidant activity of the blood to a greater extent than the use of FO.

1. Arazi H., Eghbali E., Suzuki K. Creatine Supplementation, Physical Exercise and Oxidative Stress Markers: A Review of the Mechanisms and Effectiveness // Nutrients. 2021;13(3):869. DOI: 10.3390/nu13030869.

2. Pingitore A., Lima G.P., Mastorci F. et al. Exercise and oxidative stress: potential effects of antioxidant dietary strategies in sports // Nutrition. 2015;31(7–8):916‒922. DOI: 10.1016/j.nut.2015.02.005.

3. VanDusseldorp T. A., Escobar K. A., Johnson K. E. et al. Impact of varying dosages of fish oil on recovery and soreness following eccentric exercise // Nutrients. 2020;12(8):2246. DOI: 10.3390/nu12082246.

4. Visconti L. M., Cotter J. A., Schick E. E. et al. Impact of varying doses of omega-3 supplementation on muscle damage and recovery after eccentric resistance exercise // Metabol Open. 2021;12:100133. DOI: 10.1016/j.metop.2021.100133.

5. Mackay J., Bowles E., Macgregor L. J. et al. Fish oil supplementation fails to modulate indices of muscle damage and muscle repair during acute recovery from eccentric exercise in trained young males // Eur J Sport Sci. 2023;23(8):1666‒1676. DOI: 10.1080/17461391.2023.2199282.

6. Shidfar F., Keshavarz A., Hosseyni S. et al. Effects of omega-3 fatty acid supplements on serum lipids, apolipoproteins and malondialdehyde in type 2 diabetes patients // East Mediterr Health J. 2008;14(2):305–313.

7. Toorang F., Djazayery A., Djalali M. Effects of omega-3 fatty acids supplement on antioxidant enzymes activity in type 2 diabetic patients // Iran. J. Public Health. 2016;45: 340–345.

8. Valk E. E., Hornstra G. Relationship between vitamin E requirement and polyunsaturated fatty acid intake in man: a review // Int J Vitam Nutr Res. 2000;70(2):31–42. DOI: 10.1024/0300-9831.70.2.31.

9. Deryugina A. V., Boyarinov G. A., Simutis I. S. et al. Correction of metabolic indicators of erythrocytes and myocardium structure with ozonized red blood: cell mass // Cell tissue biol. 2018;60(2):89–95. (In Russ.). DOI: 10.31116/ tsitol.2018.02.03.

10. Golubeva M. G. Influence of exercise on the functional state of erythrocytes membranes // Sports medicine: research and practice. 2020;10(2):55–64. (In Russ.).

11. Criegee R. Mechanism of Ozonolysis // Angew. Chem. Int. Ed. Engl. 1975;14:745–752. DOI: 10.1002/anie.197507451.

12. Zanardi I., Travagli V., Gabbrielli A. et al. Physico-chemical characterization of sesame oil derivatives // Lipids. 2008;43(9):877–886. DOI: 10.1007/s11745-0083218-x.

13. Peretyagin S. P., Gordetsov A. S., Solovyova A. G. et al. The influence of low-frequency electric pulse exposure on the physicochemical parameters and biological activity of a cream containing reactive oxygen species // Bioradikaly i antioksidanty. 2017;4(4):57–64. (In Russ.).

14. Livshits V. M., Sedelnikova V. I. Meditsinskiy laboratorno-analiticheskiy spravochnik. M.: Triada Kh, 2007. (In Russ.).

15. Deryugina A. V., Abaeva О. Р., Romanov S. V. et al. Electrokinetic, oxidative and aggregation properties of erythrocytes in the postoperative period during kidney transplantation // Bulletin of Transplantology and Artificial Organs. 2020;22(2):72–79. (In Russ.). DOI: 10.15825/1995-11912020-2-72-79.

16. Volchegorskiy I. A., Nalimov A. G., Yarovinskiy B. G. et al. Comparison of different approaches to the determination of lipid peroxidation products in heptane-isopropanol blood extracts // Voprosy meditsinskoy khimii. 1989;35(1):127– 131. (In Russ.).

17. Danilova D. A., Brichkin Yu. D., Medvedev A. P. et al. Application of molecular hydrogen in heart surgery under cardiopulmonary bypas // Sovremennye tehnologii v medicine. 2021;13(1):71–77. (In Russ.). DOI: 10.17691/stm2021.13.1.09.

18. Gоth L. A simple method for determination of serum catalase activity and revision of reference range // Clin Chim Acta. 1991;196(2‒3):143–51.

19. Scassellati C., Galoforo A. C., Bonvicini C. et al. Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders // Ageing Res Rev. 2020;63:101138. DOI: 10.1016/j.arr.2020.101138.

20. Aliev S. A. Influence of intensive physical loads on oxidative stress and antioxidant changes in the body of athletes // Kronos: Natural and Technical Sciences. 2020;2:17‒22.

21. Blinova T. V., Strakhova L. A., Kolesov S. A. Influence of intensive physical loads on biochemical indices of antioxidant defense and nitric oxide in swimmers // Occupational Medicine and Industrial Ecology. 2019;59:860‒864.

22. Vezzoli A., Pugliese L., Marzorati M. et al. Timecourse changes of oxidative stress response to high-intensity discontinuous training versus moderate-intensity continuous training in masters runners // PLoS One. 2014;9(1):e87506. DOI: 10.1371/journal.pone.0087506.

23. Kolupaeva E. A., Belyaeva L. M. Modern view of fish oil // Medical News. 2013;10(229):40‒42. (In Russ.).

24. Makarova V. G., Yakusheva E. N., Pravkin S. K. Vitamin A concentration, parameters of POL and antioxidant defense in experimental hepatozo-hepatitis // Russian Medical and Biological Bulletin named after Academician I. P. Pavlov. 2006;4:25‒31. (In Russ.).

25. Kulina E. V., Smolina Y. A., Osmanov I. M. et al. The role of omega-3 fatty acids in progressive kidney disease // Russian Herald of Perinatology and Pediatrics. 2016;4:81‒86. (In Russ.).

26. Lamazhapova G. P., Zhamsaranova S. D., Syngeeva E. V. Influence of polyunsaturated fatty acids concentrate on oxidative stress indicators in experimental dyslipidemia // Bulletin of Buryat State University. Medicine and pharmacy. 2017;2:6‒12. (In Russ.).

27. Struchkov A. A., Belyanina N. A., Zhiltsova O. E. et al. Study of the bactericidal effect of ozonized fish oil // Bioradicals and antioxidants. 2021;8(3):43‒49. (In Russ.).

28. Kovalchuk L. S. Biological and biochemical bases of ozone therapy // Problems of health and ecology. 2007; 2:93‒101. (In Russ.).

29. Osipov B. B., Kozlova A. E. Effect of ozone therapy on the indicators of oxidative stress and antioxidant mechanisms in experimental liver cirrhosis // Vestnik VSMU. 2018;17(1):34‒42.

30. Travagli V., Iorio E. L. The biological and molecular action of ozone and its derivatives: state-of-the-art, enhanced scenarios, and quality insights // Int J Mol Sci. 2023; 24(10):8465. DOI: 10.3390/ijms24108465

31. Yadav A., Kumari R., Yadav A. et al. Antioxidants and its functions in human body ‒ A review // Res. Environ. Life Sci. 2016;9(11):1328–1331.