Stool-based PCR test for colorectal cancer screening

Abstract

The research represents working out of non-invasive colorectal cancer screening based on DNA integrity testing of patient's fecal. Polymerase chain reaction method and the following polyacrylamide gel electrophoresis in fecal of patient with colorectal cancer detected long DNA fragments which size was 800 n.p. But the same stool DNA testing of control group detected just short DNA fragments which size was less than 200 n.p. The sensitivity and specificity of the analysis was 71 (р<0,0001) и 100 % (р<0,0001).