uzspbgmuУчёные записки Первого Санкт-Петербургского государственного медицинского университета имени академика И. П. ПавловаThe Scientific Notes of the Pavlov University1607-41812541-8807Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1607-4181-2020-27-1-75-85uzspbgmu-702Research ArticleОРИГИНАЛЬНЫЕ РАБОТЫORIGINAL PAPERSИММУНОГИСТОХИМИЧЕСКОЕ ИССЛЕДОВАНИЕ БЕЛКОВ PROX1, CD133 И CD38 В ГЛИОБЛАСТОМАХ ВЕНТРИКУЛЯРНО-СУБВЕНТРИКУЛЯРНОЙ ЗОНЫ И ИХ ВЛИЯНИЕ НА ПРОДОЛЖИТЕЛЬНОСТЬ ЖИЗНИ ПАЦИЕНТОВIMMUNOHISTOCHEMICAL STUDY OF PROTEINS PROX1, CD133 AND CD38 IN GLIOBLASTOMAS OF THE VENTRICULARSUBVENTRICULAR ZONE AND THEIR EFFECT ON THE LIFE EXPECTANCY OF PATIENTShttps://orcid.org/0000-0002-5252-483XГальковскийБ. Э.GalkovskyB. E.

врач-патологоанатом, очный аспирант 2-го года обучения ИМО

197341, Россия, Санкт-Петербург, ул. Аккуратова, д. 2

pathologist, PhD student

2, Akkuratova str., Saint Petersburg, 197341, Russia

mrc4se@gmail.comhttps://orcid.org/0000-0003-0735-7822МитрофановаЛ. Б.MitrofanovaL. B.

доктор медицинских наук, профессор кафедры патологии ИМО, главный научный сотрудник НИЛ патоморфологии

197341, Россия, Санкт-Петербург, ул. Аккуратова, д. 2

Dr. of Sci. (Med.), Professor of the Department of Pathology, Chief Researcher of the Scientific Research Laboratory of Pathomorphology

2, Akkuratova str., Saint Petersburg, 197341, Russia

lubamitr@yandex.ruhttps://orcid.org/0000-0002-5454-6397ЛахинаЮ. С.LakhinaIu. S.

Врач-лаборант НИЛ интегративных нейрохирургических технологий

197341, Россия, Санкт-Петербург, ул. Аккуратова, д. 2

laboratory assistant at the Research Laboratory of Integrative Neurosurgical Technologies

2, Akkuratova str., Saint Petersburg, 197341, Russia

highway25@yandex.ruhttps://orcid.org/0000-0002-5509-5612ГуляевД. А.GulyaevD. A.

доктор медицинских наук, руководитель НИЛ интегративных нейрохирургических технологий, врач-нейрохирург

197341, Россия, Санкт-Петербург, ул. Аккуратова, д. 2

Dr. of Sci. (Med.), Head of the Research Laboratory of Integrative Neurosurgical Technologies, neurosurgeon

2, Akkuratova str., Saint Petersburg, 197341, Russia

gulyaevd@mail.ruhttps://orcid.org/0000-0002-0610-1131ЧиркинВ. Ю.ChirkinV. Y.

Кандидат медицинских наук, врач-нейрохирург

197341, Россия, Санкт-Петербург, ул. Аккуратова, д. 2

Cand. of Sci. (Med.), neurosurgeon

2, Akkuratova str., Saint Petersburg, 197341, Russia

9831766@mail.ruhttps://orcid.org/0000-0002-9462-9226МитрофановН. А.MitrofanovN. A.

кандидат медицинских наук, доцент кафедры рентгенологии и радиационной медицины, врач-рентгенолог

 

Cand. of Sci. (Med.), associate Professor of the Department of Radiology and Radiation Medicine

 Saint Petersburg

doc_nik@mail.ruhttps://orcid.org/0000-0003-3307-0083ЧистоваИ. В.ChistovaI. V.

кандидат медицинских наук, ассистент кафедры неврологии им. акад. С. Н. Давиденкова, врач-невролог

 

Cand. of Sci. (Med.), Assistant of the Department of Neurology

Saint Petersburg

ingachistova@yandex.ruФГБУ «НМИЦ им. В. А. Алмазова» Минздрава РоссииРоссияAlmazov National Medical Research CentreRussian FederationПервый Санкт-Петербургский государственный медицинский университет им. И.П. ПавловаРоссияPavlov UniversityRussian FederationГБОУ ВПО "СЗГМУ им. И.И. Мечникова" Минздрава РоссииРоссияNorth-Western State Medical University named after I. I. MechnikovRussian Federation2020150120202717585Copyright © Гальковский Б.Э., Митрофанова Л.Б., Лахина Ю.С., Гуляев Д.А., Чиркин В.Ю., Митрофанов Н.А., Чистова И.В., 20202020Гальковский Б.Э., Митрофанова Л.Б., Лахина Ю.С., Гуляев Д.А., Чиркин В.Ю., Митрофанов Н.А., Чистова И.В.Galkovsky B.E., Mitrofanova L.B., Lakhina I.S., Gulyaev D.A., Chirkin V.Y., Mitrofanov N.A., Chistova I.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sci-notes.ru/jour/article/view/702Введение

Введение. Глиобластома (ГБМ) – одна из самых злокачественных опухолей. На текущий момент варианты лечения ГБМ ограничены, а показатели выживаемости пациентов за последние десятилетия значительно не изменились.

Цель – сравнительное изучение экспрессии белков Prox1, CD38 и CD133 в ГБМ, контактирующих (ВСВЗ+) и не контактирующих с вентрикулярно-субвентрикулярной зоной (ВСВЗ–).

Методы и материалы

Методы и материалы. Иммуногистохимическое исследование с антителами к Prox1, Ki-67, СD38, СD133 и морфометрический анализ фрагментов ГБМ 10 пациентов ВСВЗ+ и 8 пациентов ВСВЗ–.

Результаты

Результаты. Медиана числа CD133+-клеток в ВСВЗ+ и ВСВЗ– ГБМ составила 34,5 и 10 % соответственно. CD38+- клетки были обнаружены во всех ВСВЗ+ ГБМ и только в 1 ВСВЗ– ГБМ. PROX1 экспрессировался в 34,6 % клеток ВСВЗ– ГБМ и в 79 % ВСВЗ+ ГБМ. Медиана продолжительности жизни была статистически значимо больше в группе ГБМ ВСВЗ–, чем в ГБМ ВСВЗ+ (6 против 4 месяцев). Были обнаружены прямые корреляционные связи между числом CD38+- и СD133+-клеток (r=0,596), между локализацией опухоли и числом CD133+-клеток (r=0,760), обратные корреляционные связи между продолжительностью жизни и локализацией опухоли (r=–0,607), а также числом CD38+- (r=–0,755) и CD133+-клеток (r=–0,630). При проведении регрессионного анализа числа CD133+-клеток было связано с продолжительностью жизни линейной функцией.

Выводы

Выводы. ГБМ ВСВЗ+ имеют больше CD133+- и CD38+-клеток и обладают меньшей медианой выживаемости по сравнению с ГБМ ВСВЗ–.

Introduction

Introduction. Glioblastoma (GBM) is one of the most malignant tumors. Currently, treatment options for GBM are limited, and patient survival rates over the past decades have not changed significantly.

The objective of the study was a comparative study of the expression of proteins Prox1, CD38, and CD133 in GBM in contact (VSVZ+) and not in contact with the ventricular-subventricular zone (VSVZ–).

Methods and materials

Methods and materials. Immunohistochemical study with antibodies to Prox1, Ki-67, CD38, CD133 and morphometric analysis of GBM fragments of 10 patients of VSVZ + and 8 patients of VSVZ-.

Results

Results. The median of the number of CD133+ cells in VSVZ+ and VSVZ-GBM was 34.5 % and 10 %, respectively. CD38+ cells were found in all of the VSVZ+ GBM and only in one of the VSVZ– GBM. PROX1 was expressed in 34.6 % of VSBZ– GBM cells and in 79 % of VSVZ+ GBM cells. The median life expectancy was statistically significantly greater in the GBM VSVZ– group than in the GBM VSVZ+ group (6 versus 4 months). Direct correlations were found between the number of CD38+ and CD133+ cells (r=0.596), between tumor localization and the number of CD133+ cells (r=0.760), inverse correlations between life expectancy and tumor localization (r=–0.607), and the number of CD38+ (r=–0.755) and CD133+ cells (r=–0.630). Regression analysis showed that the number of CD133+ cells was associated with a lifespan of linear function.

Conclusions

Conclusions.VSVZ+ GBM have more CD133+ and CD38+ cells and have a lower median survival compared to VSVZ– GBM.

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The authors declare that there are no conflicts of interest present.