uzspbgmuУчёные записки Первого Санкт-Петербургского государственного медицинского университета имени академика И. П. ПавловаThe Scientific Notes of the Pavlov University1607-41812541-8807Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1607-4181-2019-26-2-18-27uzspbgmu-585Research ArticleОРИГИНАЛЬНЫЕ РАБОТЫORIGINAL PAPERSПродукция белков L1 и NuMA1 вируса папилломы человека при цервикальной интраэпителиальной неоплазииExpression of human papillomavirus L1 protein and NuMA1 at cervical intraepithelial neoplasiaЕршовВ. А.ErshovV. A.

Ершов Владимир Анатольевич –  заведующий цитоогической лабораторией ГКОД 

197022, Санкт-Петербург, 2-я Березовая аллея, д. 3/5. 

ershov@gkod.spb.ruЛисянскаяА. С.LisyanskayaA. S.МанихасГ. М.ManikhasG. M.Санкт-Петербургское государственное бюджетное учреждение здравоохранения «Городской клинический онкологический диспансер»РоссияMunicipal Clinical Oncology DispensaryRussian FederationСанкт-Петербургское государственное бюджетное учреждение здравоохранения «Городской клинический онкологический диспансер»; Федеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет имени академика И. П. Павлова» Министерства здравоохранения Российской ФедерацииРоссияMunicipal Clinical Oncology Dispensary; Pavlov UniversityRussian Federation2019050420192621827Copyright © Ершов В.А., Лисянская А.С., Манихас Г.М., 20192019Ершов В.А., Лисянская А.С., Манихас Г.М.Ershov V.A., Lisyanskaya A.S., Manikhas G.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sci-notes.ru/jour/article/view/585Введение

Введение. Рак шейки матки – одно из наиболее часто встречающихся среди женщин злокачественных новообразований. Ему предшествуют интраэпителиальные изменения, которые могут исчезать спонтанно или прогрессировать к раку. На настоящий момент нет маркеров, характеризующих исход цервикальных интраэпителиальных неоплазий. 

Цель исследования – изучение информативности белков L1 и NuMA1 в качестве маркеров прогноза цервикальных интраэпителиальных неоплазий, ассоциированных с вирусом папилломы человека (ВПЧ) высокого канцерогенного риска (ВКР).

Материал и методы

Материал и методы. Биоптаты шейки матки 178 женщин с цервикальными неоплазиями, ассоциированными с ВПЧ ВКР, исследованы цитологическим, гистологическим, иммуноцитохимическим методами и методом полимеразной цепной реакции.

Результаты

Результаты. Верифицированы ВПЧ ВКР-позитивные слабая (42,7 %), умеренная (34,27 %), тяжелая (21,91 %) формы дисплазии, Ca in situ (1,12 %). В 81,13 % исследований CIN с ядерной экспрессией L1 и NuMA1 отметили регресс дисплазии, в 13,21 % – сохранение степени тяжести поражения плоского эпителия, в 5,66 % – прогрессирование дисплазии. В 73,33 % наблюдений CIN с экспрессией только NuMA1 плоский эпителий восстановил типичное строение, в 26,67 % явления атипии были сохранены. В 45,45 % CIN с экспрессией только L1 отметили регресс клеточных поражений, в 48,48 % – персистенцию, в 6,06 % – прогрессирование неоплазии. Регресс или прогрессирование CIN с экспрессией L1 и NuMA1 или одного из этих белков впервые обнаружены спустя 6 месяцев после выявления клеточных изменений. 

Заключение

Заключение. Интраэпителиальные неоплазии шейки матки могут завершаться регрессом, персистенцией или прогрессированием. При экспрессии атипичными клетками L1 и NuMA1 отмечено наибольшее число – 81,13 % – случаев регресса CIN. При экспрессии атипичными клетками только белка NuMA1 CIN завершились регрессом или длительной персистенцией. Течение CIN с экспрессией белка L1 характеризовалось наибольшими показателями персистенции и прогрессирования, отмечаемых, соответственно, в 48,48 и 6,06 % случаев.

Relevance

Relevance. Cervical cancer – one of malignant new growths most often met among women. Intraepithelial changes precede to it; these changes can disappear spontaneously or progress to cancer. For the present moment, there are no markers describing the outcome of cervical intraepithelial neoplasia.

The objective was to research the expression L1 HPV and NuMA1 as factors of prognosis HPV-positive cervical intraepithelial neoplasias by high-risk human papillomavirus.

Material and methods

Material and methods. The biopsies of 178 women from HPV-positive cervical neoplasias were studied by cytological, histological, immunocytochemical methods and PCR.

Results

Results. We verified HPV-HR-positive: mild (42.7 %), moderate (34.27 %), severe (21.91 %) dysplasias, Ca in situ (1.12 %). In 81.13 % of researches, CIN with expression of L1 and NuMA1 had regression of dysplasia, in 13.21 % – persistence of grade squamous intraepithelial lesion, in 5.66 % – progression of dysplasia. In 73.33 % of cases, CIN with expression of NuMA1 had regression, in 26.67 % – persistence of dysplasia. In 45.45 % of researches, CIN with expression of L1 had regression of dysplasia, in 48.48 % – persistence of grade squamous intraepithelial lesion, in 6.06 % – progression of dysplasia. Regression or progression of dysplasia with expression L1 and NuMA1 or one of these proteins for the first time was revealed later 6 months.

Conclusion

Conclusion. CIN could come to the end with regression, persistence or progression. At expression of atypical cells L1 and NuMA1, the greatest quantity – 81.13 %, of cases of CIN regression was noted. At expression of atypical cells only NuMA1, CIN came to the end with regression or long persistence. Course of CIN with expression L1 HPV was characterized by the greatest parameters of persistence and progression marked, accordingly, in 48.48 and 6.06 % of cases.

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The authors declare that there are no conflicts of interest present.