uzspbgmuУчёные записки Первого Санкт-Петербургского государственного медицинского университета имени академика И. П. ПавловаThe Scientific Notes of the Pavlov University1607-41812541-8807Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1607-4181-2013-20-4-59-61uzspbgmu-201Research ArticleОРИГИНАЛЬНЫЕ РАБОТЫORIGINAL PAPERSПРАКТИЧЕСКИЙ ЭФФЕКТ ОТ ВНЕДРЕНИЯ КОЛОРИМЕТРИЧЕСКОЙ И ИММУНОХИМИЧЕСКОЙ МЕТОДИК ПРИ УСТАНОВЛЕНИИ НАЛИЧИЯ СПЕРМЫ НА ВЕЩЕСТВЕННЫХ ДОКАЗАТЕЛЬСТВАХPractical effect of introduction of colorimetric and immuno chemical techniques at the establishment of presence of sperm on material evidencesСидоровВ. Л.SidorovV. L.ЯгмуровО. Д.YagmurovO. D.Первый Санкт-Петербургский государственный медицинский университет имени академика И.П. ПавловаРоссия2013301220132045961Copyright © Сидоров В.Л., Ягмуров О.Д., 20132013Сидоров В.Л., Ягмуров О.Д.Sidorov V.L., Yagmurov O.D.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sci-notes.ru/jour/article/view/201

The authors analyze the experience of both domestic and of foreign authors in application of the methods for detection of rough and demonstrative presence of the sperm in the stains on the material evidences. Colorimetric method for definition of the acid phosphotase presence and the ELISA technique for definition of PSA are compared with other modern techniques applied in Russia. Sensitivity, objectivity, actuality, economyand other potentialities and advantages of both methods are more pronounced in forensic medicine practice.

иммуноферментный анализве щественные доказательствапятна спермыкислая фосфатаза.ELISAmaterial evidencessemen stainsacid phosphataseReferences/ Туманов А.К. Судебно-медицинское исследование вещественных доказательств. -М.: Госюриздат, 1961. -576 с./ Туманов А.К. Судебно-медицинское исследование вещественных доказательств. -М.: Госюриздат, 1961. -576 с.Чарный В.И. Об ориентировочном значении количественной реакции на кислую фосфатазу при установлении наличия спермы в пятнах//Судебно-мед. экспертиза. -1965. -Т. 8. -№ 2. -С. 18-22.Чарный В.И. Об ориентировочном значении количественной реакции на кислую фосфатазу при установлении наличия спермы в пятнах//Судебно-мед. экспертиза. -1965. -Т. 8. -№ 2. -С. 18-22.Collins K.A., Rao P.N., Hayworth R. et al. Identification of sperm and non-sperm male cells in cervicovaginal smears using fluorescence in situ hybridization: application in alleged sexual assault cases//J. Forensic Sci. -1994. -Vol. 39. -№ 6. -P. 1347-1355.Collins K.A., Rao P.N., Hayworth R. et al. Identification of sperm and non-sperm male cells in cervicovaginal smears using fluorescence in situ hybridization: application in alleged sexual assault cases//J. Forensic Sci. -1994. -Vol. 39. -№ 6. -P. 1347-1355.Evers H., Heidorn F., Gruber C. et al. Investigative strategy for the forensic detection of sperm traces//Forensic Sci. Med. Pathol. -2009. -Vol. 5. -№ 3. -P. 182-188.Evers H., Heidorn F., Gruber C. et al. Investigative strategy for the forensic detection of sperm traces//Forensic Sci. Med. Pathol. -2009. -Vol. 5. -№ 3. -P. 182-188.Findley T.P. Quantitayion of vaginal acid phosphatase and its relationship to time of coitus//Am. J. Clin. Pathol. -1977. -Vol. 68. -№ 2. -P. 238-242.Findley T.P. Quantitayion of vaginal acid phosphatase and its relationship to time of coitus//Am. J. Clin. Pathol. -1977. -Vol. 68. -№ 2. -P. 238-242.Johnson E.D., Kotowski T.M. Detection of prostate specific antigen by ELISA//J. Forensic Sci. -1993. -Vol. 38. -№ 2. -P. 250-258.Johnson E.D., Kotowski T.M. Detection of prostate specific antigen by ELISA//J. Forensic Sci. -1993. -Vol. 38. -№ 2. -P. 250-258.Levine B., Titus J.M., Moore K., Fowler D. Use of prostate specific antigen in the identification of semen in postmortem cases//Am. J. Forensic Med. Pathol. -2004. -Vol. 25. -№ 4. -P. 288-290.Levine B., Titus J.M., Moore K., Fowler D. Use of prostate specific antigen in the identification of semen in postmortem cases//Am. J. Forensic Med. Pathol. -2004. -Vol. 25. -№ 4. -P. 288-290.Lunetta P., Sippel H. Positive prostate-specific antigen (PSA) reaction in post-mortem rectal swabs: a cautionary note//J. Forensic Leg. Med. -2009. -Vol. 16. -№ 7. -P. 397-399.Lunetta P., Sippel H. Positive prostate-specific antigen (PSA) reaction in post-mortem rectal swabs: a cautionary note//J. Forensic Leg. Med. -2009. -Vol. 16. -№ 7. -P. 397-399.Romero-Montoya L., Martínez-Rodríguez H., Pérez M.A., Argüello-García R. Relationship of spermatoscopy, prostatic acid phosphatase activity and prostate-specific antigen (p30) assays with further DNA typing in forensic samples from rape cases//Forensic Sci. Int. -2011. -Vol. 20. -№ 1-3. -P. 111-118.Romero-Montoya L., Martínez-Rodríguez H., Pérez M.A., Argüello-García R. Relationship of spermatoscopy, prostatic acid phosphatase activity and prostate-specific antigen (p30) assays with further DNA typing in forensic samples from rape cases//Forensic Sci. Int. -2011. -Vol. 20. -№ 1-3. -P. 111-118.

The authors declare that there are no conflicts of interest present.