uzspbgmuУчёные записки Первого Санкт-Петербургского государственного медицинского университета имени академика И. П. ПавловаThe Scientific Notes of the Pavlov University1607-41812541-8807Academician I.P. Pavlov First St. Petersburg State Medical University10.24884/1607-4181-2023-30-2-88-97uzspbgmu-1022Research ArticleОБЗОРЫ И ЛЕКЦИИREVIEWS AND LECTURESНеинвазивный метод мониторинга функционального состояния живых клетокNoninvasive method of monitoring the functional state of living cellsПархоменкоТ. В.ParkhomenkoT. V.

Пархоменко Татьяна Васильевна, кандидат биологических наук, старший научный сотрудник научной лаборатории патоморфологии Научно-клинического центра патоморфологии

Санкт-Петербург

Parkhomenko Tatyana V., Dr. of Sci. (Biol.), Senior Research Fellow of the Scientific Laboratory of Pathomorphology, Scientific-Clinical Center of Pathomorphology

Saint Petersburg

ГалибинО. В.GalibinO. V.

Галибин Олег Всеволодович, доктор медицинских наук, профессор, ведущий научный сотрудник лаборатории генной и клеточной терапии отдела биотехнологий Научно-исследовательского института детской онкологии, гематологии и трансплантологии имени Р. М. Горбачёвой

Санкт-Петербург

Galibin Oleg V., Dr. of Sci. (Med.), Professor, Leading Research Fellow of the Laboratory of Gene and Cell Therapy of the Biotechnology Department, R.M. Gorbacheva Research Institute of Pediatric Oncology, Hematology and Transplantology

Saint Petersburg

ТомсонВ. В.TomsonV. V.

Томсон Владимир Викторович, доктор медицинских наук, профессор, профессор кафедры патологической анатомии с патологоанатомическим отделением

197022, Санкт-Петербург, ул. Льва Толстого, д. 6-8

Tomson Vladimir V., Dr. of Sci. (Med.), Professor, Professor of the Department of Pathological Anatomy with the Pathology Department

6-8 L’va Tolstogo str., Saint Petersburg, 197022

nic.spb@mail.ruФедеральное государственное бюджетное образовательное учреждение высшего образования «Первый Санкт-Петербургский государственный медицинский университет имени академика И. П. Павлова» Министерства здравоохранения Российской ФедерацииРоссияPavlov UniversityRussian Federation2023051220233028897Copyright © Пархоменко Т.В., Галибин О.В., Томсон В.В., 20232023Пархоменко Т.В., Галибин О.В., Томсон В.В.Parkhomenko T.V., Galibin O.V., Tomson V.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.sci-notes.ru/jour/article/view/1022

Статья посвящена обобщению сведений об использовании потенциал-чувствительных зондов (ПЧЗ) для оценки жизнеспособности и функционального состояния целой клетки. Применение синтетических зондов, чувствительных к мембранному потенциалу, имеет большое значение для мониторинга жизнеспособности и оценки функционального состояния донорских клеток и их структурных компонентов (митохондрий, ядер, цитоплазматических мембран, ионных каналов) до трансплантации, в ходе их хранения и при культивировании. Потенциальное преимущество такого подхода состоит в сохранении целостности донорских клеток. ПЧЗ позволяют оценивать состояние клеточной биоэнергетики, т. е. – баланс между продукцией и потреблением энергии в живых клетках. Продукция энергии в структурах митохондрий обеспечивает жизнеспособность клеток, а ее нарушения ведут к развитию различных заболеваний и старению. В клинической медицине этот метод может быть использован для оценки состояния донорских клеток перед их трансплантацией прежде всего в онкогематологии, лечении больных с тяжелыми ишемическими поражениями миокарда.

Цель – изучение результатов исследования применения потенциал-чувствительных зондов для оценки энергетического потенциала и жизнеспособности клеток.

The article is devoted to the generalization of information on the use of potential-sensitive probes (PSP) to assess the viability and functional state of an entire cell. Usage of membrane PSP is of great importance for assessing both the viability and functional integrity of the cells and their structural components (mitochondria, nuclei, cytoplasmic membranes, ion channels). Potential advantage of this approach includes studies of native viable cells in order to assess functional state of donor hematopoietic cells before transplantation as well as upon their storage and cultivation. These staining tools allow to assess the state of cellular bioenergetics, i.e., the balance between production and consumption of energy in living cells. The production of energy in mitochondrial structures ensures the cell viability, whereas its impairment leads to the development of different disorders and aging. In clinical medicine, this method can be used to assess the condition of donor cells before their transplantation, primarily in oncohematology, the treatment of patients with severe ischemic myocardial lesions.

The purpose of the work: to study the results of the research of the use of PSP to assess the energy potential and viability of cells.

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The authors declare that there are no conflicts of interest present.